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为了探讨急性白血病的免疫表型特征与诊断价值 ,采用流式细胞术三色免疫荧光直接标记技术检测白血病患者 ,根据抗体积分系统进行分型。结果显示 ,三染色标记法检测 180例白血病患者中 ,急性淋巴细胞白血病(ALL)患者除均表达淋系抗原外 ,同时有 4 9.4 %伴髓系抗原 (CD13,CD33)的表达 ;急性髓细胞白血病 (AML)患者除均表达髓系抗原外 ,同时有 4 3.2 %伴淋系抗原 (CD4 ,CD7)表达。结论 :流式细胞仪三色免疫荧光直接标记法进行白血病免疫分型能确诊一些特殊类型的白血病 ,对于急性白血病的诊断治疗和预后判断均有很大帮助。
In order to investigate the immunophenotypic characteristics and diagnostic value of acute leukemia, three-color immunofluorescence direct flow cytometry was used to detect leukemia patients, and the typing was based on antibody integration system. The results showed that in addition to all the lymphoid antigens, there were 44.4% with myeloid antigens (CD13, CD33) in acute leukemia patients (ALL) in 180 patients with leukemia. The number of acute myeloid leukemia In addition to myeloid antigens, 42% of leukemia (AML) patients were accompanied with lymphoid antigen (CD4, CD7) expression. Conclusion: Flow cytometry three-color immunofluorescence direct labeling method for leukemia immunophenotyping can diagnose some special types of leukemia, for the diagnosis and treatment of acute leukemia and prognosis are very helpful.