初步探讨空间环境对黑色素瘤B16细胞增殖及免疫原性的影响。选择4株经体外实验筛选出生物学性状变异明显的第20颗返回式卫星搭载的空间培养B16细胞,检测其体内增殖能力及免疫原性的变化。将筛选出的4株空间培养B16细胞接种于C57BL/6小鼠,其中一组接种于腹腔,观察荷瘤小鼠生存期;另一组接种于腋下皮肤,检测小鼠出瘤时间。接种至2周时,处死,分离荷瘤小鼠移植瘤和血清。称重荷瘤小鼠移植瘤,观察空间培养B16细胞增殖能力;利用HE染色法观察荷瘤小鼠移植瘤切片细胞形态;采用放射免疫分析法(Radioimmunoassay,RIA)测定荷瘤小鼠血清白介素-2(InterLeukin-2,IL-2)浓度,观察空间培养B16细胞免疫原性变化情况。与对照细胞相比,1株空间培养B16细胞的荷瘤小鼠生存期明显缩短,出瘤时间明显延长。HE结果显示:此株空间培养B16肿瘤组织内有淋巴细胞浸润;RIA结果显示:3株空间培养B16细胞荷瘤小鼠血清IL-2浓度明显增加。空间环境的复合因素可诱导B16细胞增殖能力及免疫原性产生变化。 Preliminary study of space environment on melanoma B16 cells proliferation and immunogenicity. Four in vitro experiments were performed to screen the B16 cells cultured in space by the 20th satellite with significant variation of biological characters. The proliferation and immunogenicity of B16 cells were detected. The four strains of B16 cells were inoculated into C57BL / 6 mice. One group was inoculated intraperitoneally to observe the survival of tumor-bearing mice. The other group was inoculated on the skin of underarm and the mice were killed. At 2 weeks after inoculation, mice were sacrificed and the tumor-bearing mice and serum were separated. The tumor-bearing mice were weighed and the proliferation of B16 cells was observed. The morphological changes of tumor-bearing mice were observed by HE staining. Radioimmunoassay (RIA) was used to detect the expression of serum interleukin-2 (InterLeukin-2, IL-2) concentrations observed in space-cultured B16 cells immunogenicity changes. Compared with the control cells, the tumor-bearing mice of one strain of B16 cells were significantly shorter in survival time and significantly longer in the time of tumorigenesis. The results of HE showed that there was lymphocytic infiltration in B16 tumor cells in this strain. The RIA results showed that the concentration of IL-2 in serum was significantly increased in the 3 B16 tumor-bearing mice. The complex factors of space environment can induce the proliferation of B16 cells and the changes of immunogenicity.