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为研究尾悬吊模拟失重大鼠肺组织细胞凋亡调控基因的变化 ,采用尾悬吊模拟失重 ,分为悬吊 7天和 2 1天及相应对照 4组 ,每组 10只健康雄性SD大鼠 ,并采用原位杂交技术检测肺组织bcl 2 /bax的表达情况。发现 7天尾悬吊模拟失重大鼠肺组织bcl 2mRNA水平明显低于对照组 ,两者之间差异有显著性意义 (P <0 0 5 ) ,但 2 1天bcl 2mRNA水平与对照组相比无显著性差异 (P >0 0 5 ) ;7天尾悬吊模拟失重大鼠肺组织baxmRNA水平明显高于对照组 ,两者之间差异有显著性意义(P <0 0 5 ) ,但 2 1天baxmRNA水平与对照组相比亦无显著性差异 (P >0 0 5 )。提示尾悬吊 7天模拟失重大鼠肺组织细胞凋亡调控基因存在变化。
In order to study the changes of apoptosis-controlling genes in the lung tissue of tail-suspended rats during simulated weightlessness, tail-suspended suspension was used to simulate weightlessness. The rats were suspended for 7 days and 21 days, and the corresponding control group 4, 10 healthy male SD rats Rats and detect the expression of bcl 2 / bax in lung tissues by in situ hybridization. The results showed that the level of bcl-2mRNA in the lungs of simulated weightless rats after 7-day tail suspension was significantly lower than that of the control group (P <0.05), but the level of bcl-2 mRNA on day 21 was significantly lower than that of the control group There was no significant difference between the two groups (P> 0.05). The level of bax mRNA in the lung tissue of 7-day tail-suspended mimic rats was significantly higher than that of the control group (P <0 05) There was no significant difference in the level of bax mRNA at 1 day compared with the control group (P> 0.05). It is suggested that there is a change in the apoptosis regulatory genes of lung tissue in 7 days after tail suspension in simulated weightless rats.