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目的:建立同时测定强肾益精颗粒中王不留行黄酮苷、松果菊苷、金丝桃苷及毛蕊花糖苷等4种成分含量的方法。方法:采用HPLC法,色谱柱为Agilent TC-C18色谱柱(250 mm×4.6 mm,5μm);流动相为0.1%磷酸溶液-乙腈,梯度洗脱,流速为1.0 mL/min;检测波长为330 nm;柱温为25℃;进样量为10μL;检测时间为90 min。结果:王不留行黄酮苷、松果菊苷、金丝桃苷和毛蕊花糖苷分别在11.6~116.6μg/mL(R~2=0.999,7)、15.1~151.4μg/mL(R2=0.999,4)、6.0~60.0μg/mL(R2=0.999,5)、3.3~33.0μg/mL(R~2=0.999,8)浓度范围内与其峰面积呈良好的线性关系;王不留行黄酮苷、松果菊苷、金丝桃苷及毛蕊花糖苷平均加样回收率分别为100.1%、102.0%、99.3%和98.1%,RSD%值分别为1.33%、1.09%、1.76%和1.37%。结论:该方法结果准确、重复性好,可用于强肾益精颗粒主要成分含量控制。
OBJECTIVE: To establish a method for the simultaneous determination of 4 flavonoids, such as flavonoid glycoside, echinacoside, hyperoside and verbascoside in Qiangshenyijing granules. Methods: The mobile phase consisted of 0.1% phosphoric acid solution - acetonitrile with gradient elution at a flow rate of 1.0 mL / min on a Agilent TC-C18 column (250 mm × 4.6 mm, 5 μm) nm; column temperature was 25 ℃; injection volume was 10μL; detection time was 90 min. Results: The concentrations of flavonoid glycosides, echinacoside, hyperoside and verbascoside were 11.6-116.6μg / mL (R ~ 2 = 0.999,7), 15.1-151.4μg / mL (R2 = 0.999, 4), 6.0 ~ 60.0μg / mL (R2 = 0.999,5) and 3.3 ~ 33.0μg / mL (R ~ 2 = 0.999,8). The results showed that there was a good linear relationship between the peak area and the concentration of flavonoid glycosides The average recoveries of echinacoside, hyperoside and verbascoside were 100.1%, 102.0%, 99.3% and 98.1%, respectively. The RSD% were 1.33%, 1.09%, 1.76% and 1.37%, respectively. Conclusion: The method is accurate and reproducible, and can be used to control the main components of Qiangshen Yijing granules.