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近年来有人报道百日咳患儿T、B和无标志(N)细胞增多。作者对此进行了深入的研究。作者通过SRFC(绵羊红细胞花环形成)试验确定T细胞,用FITC标记抗体检测表面Ig,阳性者为B细胞。从淋巴总数中减去T、B细胞,以计算N细胞。用FITC标记的抗TL血清测定淋巴细胞,阳性者为前T细胞。用免疫荧光法测定淋巴细胞,表面Ig阴性、细胞浆Ig阳性者为前B细胞。其结果如下:
In recent years, it was reported in children with pertussis T, B and no signs (N) cells increased. The author conducted in-depth research. The authors identified T cells by SRFC (sheep erythrocyte rosette formation) assay and surface Ig with FITC-labeled antibody, positive for B cells. T, B cells were subtracted from the total lymph count to calculate N cells. Lymphocytes were assayed with FITC-labeled anti-TL serum and pre-T cells were positive. Lymphocytes were detected by immunofluorescence, and Ig on the surface was negative. The former was positive B cells. The result is as follows: