论文部分内容阅读
目的 :建立α 粒子诱发人支气管上皮细胞 (BEP2D)恶性转化细胞的克隆细胞系 ,研究其核型和DNA链断裂修复能力。方法 :1.5Gyα 粒子照射的BEP2D细胞传 35代后接种裸鼠成瘤 ,取出瘤细胞进行亚克隆 ,挑出单个克隆扩大培养 ,G带显示分析细胞核型 ,脉冲电场凝胶电泳法检测DNA双链断裂。结果 :亚克隆了 5个恶性转化细胞系 (RP35T 1, 2 , 4 , 5 , 6 ) ,核型基本与BEP2D细胞相近 ,但有着不同的染色体缺失 ,其中 2株细胞 (RP35T 2和RP35T 4 )多倍体高达 40 %,明显高于BEP2D细胞。恶性转化细胞RP35T 1和RP35T 4的DNA双链断裂重接修复缺陷。结论 :建立了α 粒子诱发人BEP2D恶性转化细胞的克隆细胞系 ,DNA链断裂修复缺陷可能是α 粒子致癌的一个重要特点。
OBJECTIVE: To establish a cloned cell line derived from α-particle-induced malignant transformation of human bronchial epithelial cells (BEP2D) and study its karyotype and DNA strand break repair ability. METHODS: BLL-2D cells irradiated with 1.5Gyα particles were inoculated with nude mice for 35 passages. The tumor cells were subcloned and single colonies were picked for expansion. The G-band showed the karyotype. The DNA double-stranded DNA was detected by pulse electric field gel electrophoresis fracture. RESULTS: Five malignant transformed cell lines (RP35T 1, 2, 4, 5, 6) were subcloned and their karyotypes were similar to those of BEP2D cells but with different chromosomal deletions. Two of these cells (RP35T 2 and RP35T 4) Polyploidy up to 40%, significantly higher than BEP2D cells. Defects of double stranded DNA double strand breaks in RP35T 1 and RP35T 4 malignant transformed cells. CONCLUSION: The cloned cell line of human BEP2D malignant transformed cells induced by α particle is established. The defect of DNA strand break repair may be an important feature of carcinogenesis of α particle.