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通过对CLC基因家族7个基因的蛋白序列比对,发现他们具有比较高的同源性,在玉米苗期用KNO3、Na Cl、PEG对其进行处理,分别在处理1、4、8、16、24、36 h后检测其体内NO3-、Cl-的浓度,同时取上述几个时间段处理的玉米根和叶,用q PCR检测CLC家族基因的表达量,研究玉米硝态氮吸收与CLC基因表达量之间的关系。结果表明,综合玉米中NO3-、Cl-的变化与CLC家族基因表达量的变化,明确在KNO3、Na Cl、PEG处理下CLC家族基因表达量变化的规律,BT066984.1、GQ412103.1、NM001176231.1和NM001136935.2这4个基因对硝酸盐的响应明显,很可能具有运输硝酸盐的作用。
Through the comparison of the protein sequences of seven genes of CLC gene family, they were found to have high homology and treated with KNO3, NaCl and PEG at the seedling stage of maize, , 24,36 h after the detection of its NO3-, Cl- concentration, while taking the above treatment of corn roots and leaves for several time periods, using q PCR to detect the expression of CLC family of genes to study the nitrate absorption of nitrate and CLC The relationship between the amount of gene expression. The results showed that the changes of gene expression of CLC family under the treatment of KNO3, NaCl and PEG were comprehensively determined by the changes of NO3- and Cl- in maize and the expression of CLC family genes. The results showed that the changes of gene expression of CLC family under the treatments of BT066984.1, GQ412103.1, NM001176231 The 4 genes of NM1 and NM001136935.2 respond well to nitrates and may have the potential to transport nitrates.