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目的研究荧光增白剂诱导中国仓鼠卵巢细胞微核形成和基因突变的作用,探讨荧光增白剂对体外哺乳动物细胞的遗传物质损伤机制。方法使用不同浓度的荧光增白剂28处理中国仓鼠卵巢细胞,通过体外哺乳动物细胞基因突变实验和体外哺乳动物细胞微核实验检测细胞遗传物质的损伤程度。结果 0.5556 mg/m L组和1.6667 mg/m L组hprt基因突变频率和微核率与阴性对照组无显著性差异,5.000 mg/m L组hprt基因突变频率和微核率显著高于阴性对照组(P<0.05),阳性对照组hprt基因突变频率和微核率显著高于阴性对照组(P<0.01)。结论荧光增白剂28可引起体外细胞的遗传物质损伤。
Objective To investigate the effect of fluorescent whitening agent on micronuclei formation and gene mutation in Chinese hamster ovary cells and to explore the mechanism of the fluorescent substance whitening agents on the damage of genetic material in mammalian cells in vitro. Methods Chinese hamster ovary cells were treated with different concentrations of optical brightener 28, and the degree of damage of cytoplasmic substances was detected by in vitro mammalian cell gene mutation experiments and in vitro mammalian cell micronucleus test. Results The frequency of mutation and micronucleus rate of hprt gene in 0.5556 mg / m L group and 1.6667 mg / m L group had no significant difference compared with negative control group. The mutation frequency and micronucleus rate of hprt gene in 5.000 mg / m L group were significantly higher than those in negative control Group (P <0.05). The frequencies of hprt gene mutation and micronucleus in the positive control group were significantly higher than those in the negative control group (P <0.01). Conclusion Fluorescent brightener 28 can cause genetic damage to cells in vitro.