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磷是植物体生长发育所必需的大量营养元素之一,广泛参与植物体多种生命活动。土壤中磷的有效性很低,是农业生产中限制作物产量的重要因素。OsPHR3(LOC_Os02g04640)属于MYB-CC家族,与水稻中磷信号途径中心调控因子OsPHR2是同源基因,且具有部分功能重叠。本研究利用转基因技术获得OsPHR3基因的突变体和超表达材料,通过水培实验、~(32)Pi同位素实验以及桶培实验来研究该基因在吸收利用磷素过程中的作用。水培实验表明,与野生型相比,突变体磷含量无明显差异,基因超表达能够提高水稻体内磷含量。~(32)Pi同位素实验显示,与野生型相比,缺磷时突变体吸收速率降低,而该基因超表达能够促进磷素的吸收与转运。桶培实验表明,该基因超表达能够增加水稻有效分蘖数,提高种子中磷含量,该基因缺失使得穗长变短。OsPHR3基因可能调控促进磷的吸收与向地上部转运。该研究将为以后分子育种提供依据。
Phosphorus is one of a large number of nutrients necessary for plant growth and development, and is widely involved in a variety of plant life activities. The low availability of phosphorus in soils is an important factor limiting crop yields in agricultural production. OsPHR3 (LOC_Os02g04640) belongs to the MYB-CC family and is homologous to OsPHR2, a central regulatory element of P signaling pathway in rice, and has partial functional overlap. In this study, we obtained the mutant and overexpression material of OsPHR3 gene by using transgenic technology. The effects of this gene in the process of phosphorus uptake and utilization were investigated by hydroponic experiment, ~ (32) Pi isotope experiment and barrel culture experiment. Hydroponic experiments showed that compared with the wild type, there was no significant difference in the phosphorus content of the mutant, and the overexpression of the gene could increase the phosphorus content in the rice. ~ (32) Pi isotope experiments showed that compared with the wild-type, the rate of uptake by mutants was reduced when phosphorus was depleted, while the overexpression of this gene promoted the uptake and transport of phosphorus. Barrel culture experiments showed that overexpression of this gene could increase the effective tiller number of rice and increase the content of phosphorus in seeds. The deletion of this gene shortened the spike length. The OsPHR3 gene may regulate the uptake and transport of P up-shoots. This research will provide the basis for future molecular breeding.