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目的研究家兔静脉注射左氧氟沙星(LVFX)后其血液和组织液中的药物分布及药代动力学参数,为铜绿假单孢菌及常见致病菌转入动物体内实验提供依据。方法建立兔组织笼模型,用高效液相色谱法测定给药后不同时点家兔血液和组织液的药物浓度,计算药代动力学参数。结果静脉注射左氧氟沙星(10m g/kg)后,血液、组织液峰质量浓度(Cm ax)分别为(8.27±0.9)、(2.48±0.3)m g/L,消除半衰期(T1/2β)分别为(1.06±0.1)、(3.63±1.3)h,表观分布容积(V)分别为(1.55±0.3)、(4.89±2.1)L/kg。结论 LVFX直接静脉注射给药后,能迅速到达组织液中,在组织液中存留时间长,药物浓度波动小,有助于维持组织液的有效浓度。
Objective To study the drug distribution and pharmacokinetic parameters of blood and tissue fluid after intravenous injection of levofloxacin (LVFX) in rabbits, and provide the basis for the in vivo experiments of Pseudomonas aeruginosa and common pathogens into animals. Methods Rabbit tissue cages model was established. The drug concentrations of blood and tissue fluid in rabbits at different time points after administration were determined by HPLC. Pharmacokinetic parameters were calculated. Results After intravenous injection of levofloxacin (10 m g / kg), the peak concentrations of blood and tissue fluid (Cm ax) were (8.27 ± 0.9) and (2.48 ± 0.3) mg / L respectively and the elimination half-lives (T1 / 2β) ± 0.1), (3.63 ± 1.3) h, and the apparent volume of distribution (V) were (1.55 ± 0.3) and (4.89 ± 2.1) L / kg, respectively. Conclusion LVFX directly reaches the tissue fluid after intravenous injection. It has a long residence time in tissue fluid and small fluctuation of drug concentration, which helps maintain the effective concentration of tissue fluid.