论文部分内容阅读
Hemocyte counts and phenoloxidase (PO) activity were examined after hemolymph being incubated in dopamine (DA),noradrenaline (NE) and serotonin (5-HT).Results showed that all the three biogenic amines (BAs) had a significant impact on total hemocyte count (THC),differential hemocyte count (DHC),and intracelluar and extracelluar phenoloxidase (PO) activity.Among these Bas,DA had the strongest effect on the above parameters,whereas 5-HT had the least effect.Preincubation with D1 receptor antagonist SCH23390,D2 receptor antagonist Sulpiride and 1∶1 admixture of the two could significantly inhibit the effect of DA on these parameters.SCH23390 showed a stronger inhibitory effect than Sulpiride,and the admixture exhibited the strongest effect.These results suggested that the change of hemocyte count and activation of prophenoloxidase (proPO) system in Litopenaeusvannamei hemocyte can be regulated by BAs,and DA modulates the two parameters via its receptors.