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目的检测IL-18在CCL4诱导大鼠肝纤维化形成过程中表达水平的变化,探讨IL-18是否可以作为肝硬化早期的预警信号或重要的血清学指标,为临床诊断和治疗提供理论依据。方法将48只雄性SD大鼠随机分为试验组(n=24)和正常对照组(n=24)。试验组用40%的CCL4油溶液皮下注射(0.3ml/100g),以10%的酒精为唯一饮用水;正常对照组用等剂量蒸馏水皮下注射,普水做饮用水。在给药(蒸馏水)前(即0周)及给药(蒸馏水)后2、4、6、8周将大鼠断尾采血后离心取血清用ELISA法检测IL-18的水平。结果试验组与正常对照组相比差异明显(P<0.05),有统计学意义;试验组IL-18在4周、6周与0周、2周相比(P<0.05)有统计学意义;正常对照组给蒸馏水前后IL-18的差异不显著(P>0.05),没有统计学意义。结论 IL-18对肝纤维化有预测价值。
Objective To detect the expression of IL-18 in the process of CCL4-induced hepatic fibrosis in rats, and to explore whether IL-18 can serve as an early warning signal or an important serological marker in the early stage of cirrhosis and provide a theoretical basis for clinical diagnosis and treatment. Methods 48 male SD rats were randomly divided into experimental group (n = 24) and normal control group (n = 24). The experimental group was subcutaneously injected with 40% CCL4 oil solution (0.3ml / 100g) with 10% alcohol as the only drinking water. The normal control group was injected subcutaneously with the same dose of distilled water and made into drinking water. The rats were collected by tail-tailed blood collection before serum administration (distilled water) (ie 0 week) and 2, 4, 6, and 8 weeks after administration (distilled water), and the serum was collected by ELISA. Results There was significant difference between the experimental group and the normal control group (P <0.05), and there was a significant difference between the experimental group and the control group (P <0.05) ; There was no significant difference in IL-18 between normal control group and distilled water (P> 0.05). Conclusion IL-18 has predictive value for liver fibrosis.