[Objective] The aim was to study on the variation of endogenous hormones contents in Aconitum flavum Hand.-Mazz seeds during the process of dormancy and germination. [Method] Contents of GA3, IAA, ABA and Z in new and old seeds of A. flavum, which was processed under stratification, were determined and analyzed by HPLC, respectively. The germination rate of seeds was investigated. [Result] Content of ABA in seeds declined, while that of GA3 increased during the process of stratification; different stratification treatments had certain effects on seed germination with the extension of time. [Conclusion] GA3 played an important role in breaking dormancy and promoting germination of seeds; ABA was an important inhibitor for germination; IAA played an important role in promoting seeds germination; there was no significant correlation between Z content and seed germination of A. flavum. [Objective] The aim was to study on the variation of endogenous hormones contents in Aconitum flavum Hand. -Mazz seeds during the process of dormancy and germination. [Method] Contents of GA3, IAA, ABA and Z in new and old seeds of A . flavum, which was processed under stratification, were determined and analyzed by HPLC, respectively. The germination rate of seeds was investigated. [Result] Content of ABA in seeds declined, while that of GA3 increased during the process of stratification; different stratification treatments had certain effects on seed germination with the extension of time. [Conclusion] GA3 played an important role in breaking dormancy and promoting germination of seeds; ABA was an important inhibitor for germination; IAA played an important role in promoting seeds germination; there was no significant correlation between Z content and seed germination of A. flavum.