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2010年9月—2011年10月,在山西省灵空山油松和辽东栎混交林样地采取随机区组设计,研究了地表凋落物和氮添加处理对土壤微生物生物量碳、氮和微生物活性的影响.凋落物处理包括:剔除凋落物(N)、叶凋落物加倍(L)、枝果凋落物加倍(B)和混合凋落物加倍(LB);氮添加量分别为0(N0)、5g.m-2.a-1(N1)和10g.m-2.a-1(N2).结果表明:剔除地表凋落物且无氮添加时,油松和辽东栎混交林地的土壤有机碳(SOC)含量显著降低,其他试验处理间对SOC的影响无显著差异.土壤微生物生物量碳(MBC)、氮(MBN)及其活性(MR)的变化范围依次为:262.42～873.16mg.kg-1、73.55～173.85mg.kg-1和2.38～3.68mg.kg-1.d-1.MBC、MBN和MR两两间呈极显著正相关.氮添加对MBC、MBN和MR均无显著影响;凋落物处理对MR影响显著,表现为混合凋落物加倍处理的MR最高,叶凋落物加倍处理次之,剔除凋落物处理最低,而对MBC和MBN无显著影响.凋落物和氮添加处理在整个试验过程中未表现出交互作用.短期的氮添加处理和森林地表凋落物变化对土壤微生物过程的影响有限.
From September 2010 to October 2011, a randomized block design of Pinus tabulaeformis and Quercus liaotungensis mixed forest in Lingkong Mountain of Shanxi Province was conducted to study the effects of surface litter and nitrogen addition on soil microbial biomass carbon, nitrogen and microbial activity (N), foliar litter doubled (L), branch litter doubled (B) and mixed litter doubled (LB). Nitrogen additions were 0 (N0), The results showed that when soil litter was removed and no nitrogen was added, soil organic carbon (SOC) of Pinus tabulaeformis and Quercus liaotungensis (SOC), and the other treatments had no significant difference on the SOC.Results showed that the range of MBC, MBN and MR was 262.42 ~ 873.16 mg.kg -1,73.55 ~ 173.85mg.kg-1 and 2.38 ~ 3.68mg.kg-1.d-1.MBC, MBN and MR showed a significant positive correlation between the two.Nitrogen addition had no significant effect on MBC, MBN and MR The effect of litter treatment on MR was significant, which was the highest MR of mixed litter, the second litter of leaf litter, the lowest litter removal, but no significant effect on MBC and MBN.Litter and Nitrogen addition did not show any interaction during the whole experiment, and short-term nitrogen addition and litter changes had limited impact on soil microbial processes.