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虽然强效的抗逆转录病毒的疗法可以控制HIV-1的感染,但CD_4+T细胞变成了长期存活的有传染性病毒的贮源。作者通过测定对HIV—1复制与细胞有关的病毒DNA和遗传信使RNA(mRNA)的水平对HIV贮主进行了研究。从有长期HIV感染并用强效抗逆转录病毒药物治疗期间其血浆内HIV-1 RNA已达不到复检水平的共20个月或20个月以上的5名患者,每6个月取外周血单核细胞样本进行研究。结果表明,在治疗前,血浆内HIV-1 RNA水平与在细胞内未整合的HIV-1 DNA以及非拼接的病毒mRNA的水平相关。治疗后,血浆内HIV-1 RNA的水平下降2.7log至不可检出的水平。与细胞有关的整合与未整合的HIV-1 DNA和mRNA的下降现象出现2个阶段。第一阶段出现在治疗的500天,其特征为DNA与mRNA水平有实质性下
Although potent antiretroviral therapy can control HIV-1 infection, CD4 + T cells have become sources of long-lived infectious virus. The authors studied HIV hosts by measuring levels of HIV-1-replicating cell-associated viral DNA and genetic messenger RNA (mRNA). Five patients, 20 months or more in total, who had not reached the retest level in their plasma with HIV-1 RNA during long-term HIV infection and with potent antiretroviral therapy, were enrolled every 6 months Blood mononuclear cells were studied. The results showed that plasma levels of HIV-1 RNA correlate with levels of unintegrated HIV-1 DNA in the cell and non-spliced viral mRNA levels prior to treatment. After treatment, the level of HIV-1 RNA in plasma decreased by 2.7 log to undetectable levels. There are two phases of cell-associated decline in both integrated and unconjugated HIV-1 DNA and mRNA. The first phase appeared at 500 days of treatment and was characterized by substantial sub-mRNA and mRNA levels