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目的:建立同时测定延胡索中巴马汀、脱氢紫堇碱和延胡索乙素的HPLC方法。方法:采用C18柱(4.6 mm×250nm,5μm),以乙腈(A)-0.1%磷酸(加三乙胺调节pH 6.0,B)进行梯度洗脱(0～20 min,25%A;20～30 min,25%～55%A;30～45 min,55%A;45～50 min,55%～25%A;50～55 min,25%A);检测波长为270,280 nm,柱温30℃。结果:巴马汀在2.12×10-2～1.59μg呈良好的线性关系(r=0.999 9);脱氢紫堇碱在4.10×10-2～3.07μg呈良好的线性关系(r=0.999 9);延胡索乙素在2.10×10-2～1.57μg呈良好的线性关系(r=0.999 9);加样回收率分别为100.10%,100.03%,97.47%。结论:该方法准确、稳定、分离效果好,可用于测定延胡索药材中巴马汀、脱氢紫堇碱和延胡索乙素的含量。
Objective: To establish a HPLC method for the simultaneous determination of palmatine, dehydro-cinerate and tetrahydropalmatine in Corydalis yanhusuo. METHODS: The elution was performed on a C18 column (4.6 mm × 250 nm, 5 μm) with gradient elution from acetonitrile (A) to 0.1% phosphoric acid (adjusted to pH 6.0 with triethylamine) (0-20 min, 25% A; 30 min, 25% -55% A, 30-45 min, 55% A, 45-50 min, 55% -25% A, 50-55 min, 25% A). The detection wavelength was 270,280 nm and the column temperature was 30 ℃. Results: There was a good linear relationship between palmatine and 2.12 × 10-2 ~ 1.59μg (r = 0.999 9). There was a good linear relationship between dehydration corydalis and 4.10 × 10-2 ~ 3.07μg (r = 0.999 9 ). The calibration curve of tetrahydropalmatine was good at 2.10 × 10-2 ~ 1.57μg (r = 0.999 9). The recoveries were 100.10%, 100.03% and 97.47% respectively. Conclusion: The method is accurate, stable, and has good separation effect. It can be used to determine the content of palmatine, dehydration corydaline and tetrahydropalmatine in Corydalis.