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目的:研究罗汉果多糖SGPS2的结构。方法:利用DEAE-纤维素柱和凝胶柱(Sephadex G-200)层析分离纯化得到多糖SGPS2,通过HPLC测定相对分子质量,用甲基化分析、部分酸水解、气相色谱分析、红外光谱、核磁共振光谱等方法研究单糖组成及连接方式。结果:SGPS2的相对分子量为650 000,它由鼠李糖和葡萄糖醛酸组成,各糖残基的摩尔比为Rha:GlcA=8.24∶0.99;SGPS2由(1→2,4)Rha和(1→4)Rha构成主链,侧链由(1→2)Rha和(1→3)Rha片段组成,Rha也为末端基;GlcA在分子中是以末端GlcA和2-位取代的GlcA的形式存在。结论:罗汉果多糖SGPS2是由鼠李糖和葡萄糖醛酸组成的杂多糖。
Objective: To study the structure of Siraitia grosvenori polysaccharide SGPS2. METHODS: The polysaccharide SGPS2 was separated and purified by chromatography on DEAE-cellulose column and Sephadex G-200 column. The relative molecular mass was determined by HPLC. Methylation analysis, partial acid hydrolysis, gas chromatography, infrared spectroscopy, NMR spectroscopy and other methods to study monosaccharide composition and connection. Results: The relative molecular weight of SGPS2 was 650 000, which consisted of rhamnose and glucuronic acid. The molar ratio of each sugar residue was Rha: GlcA = 8.24: 0.99. SGPS2 consisted of (1 → 2,4) Rha and (1 → 4) Rha constitutes the main chain, the side chains are composed of (1 → 2) Rha and (1 → 3) Rha fragments, and Rha is also a terminal group; GlcA is in the molecule in the form of GlcA substituted with terminal GlcA and 2- exist. Conclusion: The polysaccharide SGSG is a heteropolysaccharide composed of rhamnose and glucuronic acid.