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肠聚集性大肠杆菌(EAggEC)产生一种质粒介导的耐热肠毒素和一种抗原性与大肠杆菌溶血素有关的不耐热蛋白质,同时EAggEC菌株在免和大鼠回盲袢产生出血性病灶,这种病灶的出血特征与许多感染EAggEC的患儿产生的血性腹泻一致。作者应用豚鼠、兔、大鼠、绵羊和人O型血红细胞研究不同培养条件下生长的EAggEC产生的接触性溶血素(CH)的活性。方法是:将贮存菌种传代于含0.6%酵母浸膏的胰酶大豆琼脂培养基上,再转种至各种不同的肉汤(10ml)培养基中,在20～42℃振荡培养8～24h待用。用PBS(pH7.4)洗涤菌液2次,调整浓度至2×10~6cfu/ml;取菌液及红细胞(4×10~9/ml)各50μl加于U型微量
Intestinal E. coli (EAggEC) produces a plasmid-mediated heat-labile enterotoxin and an antigenic heat-labile protein associated with E. coli hemolysin, while the EAggEC strain produces hemorrhages in both free and rat ileocecal 袢The lesion, the bleeding characteristic of this lesion, is consistent with bloody diarrhea in many children infected with EAggEC. The authors used guinea pig, rabbit, rat, sheep, and human O-type red blood cells to study the activity of contact hemolysin (CH) produced by EAggEC grown under different culture conditions. The method comprises the following steps: the stored bacteria are passed on a tryptic soy agar medium containing 0.6% yeast extract, then transplanted into various broths (10 ml) 24h stand-by The bacterial solution was washed twice with PBS (pH7.4) to adjust the concentration to 2 × 10 ~ 6 cfu / ml; 50 μl of bacterial solution and red blood cells (4 × 10 ~ 9 / ml)