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作者用设计合成的两对引物建立了巢式ＰＣＲ－ＥＢ染色法检测Ｂ１９ＤＮＡ新技术，扩增产物为１０４ｂｐ片段，敏感性达０．００７ｆｇ，是一般ＰＣＲ的１００倍，是一种特异、敏感、简便、快速诊断Ｂ１９感染的新方法．用该方法检测了５７例自然流产和死胎的胎儿组织，１７例阳性，其中自然流产１１／４２（２６％），死胎６／１５（４０％），证实了国内胎儿有Ｂ１９病毒感染，和自然流产、死胎可能相关，并对ＰＣＲ实验中防污染措施进行了探讨．
The authors designed a nested PCR-EB method to detect B19 DNA by using two pairs of primers designed and synthesized. The amplified product was 104bp with a sensitivity of 0.007fg, which was 100 times of that of general PCR. It was a specific, sensitive, A Simple and Rapid Method to Diagnose B19 Infection. Fifty-seven fetuses with spontaneous abortion and stillbirth were detected by this method, of which 17 were positive, including 11/42 (26%) spontaneous abortion and 6/15 (40%) stillbirth, confirming the presence of B19 virus infection in domestic fetuses and natural Abortion and stillbirth may be related, and the anti-pollution measures in PCR experiments were discussed.