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目的建立水中微囊藻毒素-RR(MC-RR)的量子点(QDs)荧光猝灭法快速测定法。方法在p H 7.17、反应温度37℃,Cd Se-Cd S量子点浓度2.0 mg/ml条件下,将Cd Se-Cd S量子点与MC-RR单克隆抗体共价连接,形成QDs-MC-RR单克隆抗体生物共轭体。加入含MC-RR水样,测定生物共轭体的荧光强度,根据体系荧光强度与浓度的关系,求出水样中MCRR的含量。结果 MC-RR浓度在0.24~4 nmol/L的线性范围内,所得QDs-MC-RR体系的回归方程为(F0-Fn)/F0=0.110 3c+0.022 93,r=0.994 6,呈较好的线性关系,且符合Lambert-Beer公式。方法的检出限为2.4×10-10 mol/L,RSD为4.2%~9.4%,加标回收率在84.0%~90.7%之间。结论该方法具有操作更为简便快速,检测成本低,易于推广普及的优点,可以满足实际水样中MC-RR的检测需要。
OBJECTIVE To establish a rapid method of fluorescence quenching (QDs) of microcystin-RR (MC-RR) in water. Methods CdSe-CdS QDs and MC-RR monoclonal antibodies were covalently linked at p H 7.17, reaction temperature of 37 ℃ and CdSe-CdS quantum dot concentration of 2.0 mg / ml to form QDs-MC- RR Monoclonal Antibodies Bioconjugates. The MC-RR water sample was added to measure the fluorescence intensity of the bioconjugate, and the content of MCRR in the water sample was determined according to the relationship between the fluorescence intensity and the concentration of the system. Results The regression equation of QDs-MC-RR system was (F0-Fn) /F0=0.1103c +0.02293, r = 0.994 6, which showed a good linearity within the range of 0.24-4 nmol / L Linear relationship, and in line with Lambert-Beer formula. The detection limit was 2.4 × 10-10 mol / L, RSD was 4.2% ~ 9.4% and the recoveries were between 84.0% ~ 90.7%. Conclusion The method has the advantages of simple and quick operation, low detection cost and easy popularization and popularization, which can meet the detection needs of MC-RR in actual water samples.