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笔者利用国产端粒酶原位杂交检测试剂盒在大肠癌组织的石蜡切片中检测端粒酶活性 ,获得满意结果。现将应用该方法的体会简要介绍如下。1 材料与方法1.1 观察对象 手术切除的新鲜大肠癌组织 2 0例 ,对离体组织立即液氮冷冻 ,- 80℃冰箱保存。标本分为 2份 ,1份在做 5 μｍ厚
The authors used domestic telomerase in situ hybridization detection kit to detect telomerase activity in paraffin sections of colorectal cancer tissue and obtained satisfactory results. The experience of applying this method is briefly described below. 1 Materials and methods 1.1 Observing objects Surgical resection of fresh colorectal cancer tissue in 20 cases, immediately isolated liquid nitrogen freezing, -80 °C refrigerator. Specimens are divided into 2 parts, 1 part is 5 μm thick