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Mueller 1975年首先发现酸性α醋酸萘酯酶(Acid αnaphthyl acetta esterase,ANAE)能特异性地标记T淋巴细胞,这种方法用血涂片或组织切片在光学显微镜下即可鉴别T和B淋巴细胞,操作简便、快速、重复性好、特异性强,不需要特殊的条件和仪器,是识别T、B淋巴细胞技术上的一大改进,对细胞免疫学的研究和应用将起重要推动作用。现将我们所复习到的有关这方面研究文献综合介绍如下。一、ANAE 反应的原理ANAE反应的原理是细胞质内酸性α-醋酸萘酯酶能将孵有液内α醋酸萘酯水解,产生α-萘酚和醋酸离子,然后α萘酚和孵育液内的六偶氮副品红或fast garnetGBC偶联,在酶所在部位形成不溶性的棕红色沉淀物,从而对细胞起标记作用。淋巴细
Mueller first discovered that acid α-naphthyl acetta esterase (ANAE) can specifically label T lymphocytes in 1975. In this method, blood smear or tissue sections can be used to identify T and B lymphocytes under a light microscope , Easy to operate, fast, reproducible, specific, does not require special conditions and equipment, is to identify T, B lymphocyte technology a major improvement on the study and application of cellular immunology will play an important role in promoting. Now we have reviewed the research literature in this area are summarized below. First, the principle of ANAE reaction ANAE reaction principle is the cytoplasmic acidic α-naphthyl acetate esterase hatch liquid α-naphthyl acetate acetate hydrolysis, resulting α-naphthol and acetate ions, and then alpha naphthol and incubation solution Hexaazaisuban, or fast garnetGBC conjugate, forms an insoluble brown-red precipitate at the site of the enzyme, marking the cell. Lymphatic