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研究ｍａｎｏａｌｉｄｅ对除去生长因子（ａＦＧＦ和血清）而诱导的血管内皮细胞凋亡的影响．方法：通过细胞形态观察，ＤＮＡ凝胶电泳及荧光显微术等方法确定ｍａｎｏａｌｉｄｅ对细胞凋亡的抑制或促进作用．结果：向去除ａＦＧＦ和血清的培养液中加低浓度的ｍａｎｏａｌｉｄｅ（１－４μｍｏｌ·Ｌ－１），培养细胞４８ｈ，细胞的脱壁和ＤＮＡ片断化受到抑制；ｍａｎｏａｌｉｄｅ浓度为７μｍｏｌ·Ｌ－１时，促进细胞脱壁和ＤＮＡ片断化．结论：低浓度的ｍａｎｏａｌｉｄｅ（２μｍｏｌ·Ｌ－１）抑制血管内皮细胞凋亡，而较高浓度的ｍａｎｏａｌｉｄｅ（７μｍｏｌ·Ｌ－１）促进该细胞凋亡．
To investigate the effect of manoalide on the apoptosis of vascular endothelial cells induced by the removal of growth factors (aFGF and serum). Methods: Inhibition or promotion of apoptosis by manoalide was determined by cell morphology observation, DNA gel electrophoresis and fluorescence microscopy. Results: After adding low concentration of manoalide (1-4μmol·L-1) to culture medium with aFGF and serum for 48h, cell detachment and DNA fragmentation were inhibited. When manoalide concentration was 7μmol·L-1 , Promote cell wall and DNA fragmentation. CONCLUSION: Low concentration of manoalide (2μmol·L-1) inhibits vascular endothelial cell apoptosis, while higher concentration of manoalide (7μmol·L-1) promotes apoptosis.