目的了解浙江沿海地区无偿献血者人类嗜T淋巴细胞病毒(HTLV)感染状况,提出综合性防控策略。方法采用ELISA方法筛查2013-2015年浙江省沿海6市(区)无偿献血者58 609(人)份血清中抗-HTLV-Ⅰ/Ⅱ,阳性者以WB法确证,并做HTLV基因测序和同源性分析;随机对10 197名献血者开展HTLV知晓情况问卷调查。结果本组献血者人群的抗-HTLV阳性率0.01%(5/58 609),抗体类型为抗-HTLV-Ⅰ,均属世界群世界人种亚型;该5位HTLV感染者均为本地首次献血者,其中台州市发现的HTLV毒株与福建泉州的分离株同源,舟山市发现的HTLV毒株与日本的分离株MT-2同源。献血者对HTLV的知晓情况:随机问卷调查(10分制)人均得分0.75(0-8)分,其中<1分者占调查对象的79.0%(8059/10 197),>5分者占1.40%(143/10 197)。结论浙江沿海台州和舟山地区献血人群中存在HTLV感染和散在的小流行;献血者对HTLV的知识极其匮乏,亟待采取相应防控策略。 Objective To understand the status of human T-cell lymphotrophic virus (HTLV) infection in unpaid blood donors in Zhejiang coastal areas and to provide a comprehensive prevention and control strategy. Methods The anti-HTLV-Ⅰ / Ⅱ sera of 58 609 serum samples of unpaid blood donors in 6 cities (districts) of Zhejiang province from 2013 to 2015 were screened by ELISA. The positive samples were confirmed by WB method and sequenced. Homology analysis. A random sample of 10 197 blood donors was surveyed for the prevalence of HTLV. Results The positive rate of anti-HTLV was 0.01% (5/58 609) and the type of antibody was anti-HTLV-Ⅰ in the blood donors in this study. All of the 5 HTLV infected persons were local subtype Blood donors, of which HTLV strains found in Taizhou and Quanzhou, Fujian isolates homologous, HTLV strains found in Zhoushan and Japan isolates MT-2 homology. Blood donors know the situation of HTLV: random questionnaire (10 points) per capita score 0.75 (0-8) points, of which <1 points accounted for 79.0% of respondents (8059/10 197),> 5 points accounted for 1.40 % (143/10 197). Conclusion There is a small epidemic of HTLV infection and dispersal among blood donors in Taizhou and Zhoushan areas along the coast of Zhejiang Province. The donors’ knowledge of HTLV is extremely scarce, and corresponding prevention and control strategies are urgently needed.