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目的:通过动物实验,观察清热滋阴活血中药复方制剂对大鼠糖尿病牙周炎的疗效,并初步探讨其机制。方法:10周龄健康雄性Wistar大鼠随机分为健康对照组(A组)、糖尿病牙周炎组(B组)和中药治疗组(C组),B组与C组糖尿病牙周炎造模成功后,C组灌胃等效剂量的金银花、北沙参、川芎复方水煎剂,A组与B组灌胃等体积的生理盐水,2个月后,麻醉下方块截取包括实验区磨牙的双侧上颌骨,HE染色后,选择包括牙龈结缔组织及上皮组织的视野计数浸润的炎性细胞,测量实验牙近中牙槽嵴顶至釉牙骨质界的距离;腹腔总静脉抽取静脉血,制备血清后用ELISA方法检测TNF-α的含量,应用SPSS10.0软件对数据进行单因素方差分析。结果:B组与C组牙龈组织的浸润炎性细胞数目、牙槽嵴顶至釉牙骨质界的距离较A组均有增高,且B组高于C组,三组两两比较差异均具有统计学意义(P<0.05);B组外周血TNF-α的含量较A组与C组高,差异均有统计学意义(P<0.05),但A组与C组间无统计学差异(P>0.05)。结论:清热滋阴活血中药复方制剂对糖尿病牙周炎有治疗作用,可降低外周血TNF-α的水平。
OBJECTIVE: To observe the curative effect of compound prescription of clearing heat, nourishing Yin and promoting blood circulation on diabetic periodontitis by animal experiments and to explore its mechanism. Methods: Healthy male Wistar rats of 10 weeks old were randomly divided into healthy control group (A group), diabetic periodontitis group (B group) and traditional Chinese medicine treatment group (C group). Group B and group C , Group C was given gavage with equivalent dose of Honeysuckle, Radix Astragali, Ligusticum Chuanxiong decoction. Groups A and B were given the same volume of normal saline. After 2 months, the blocks under anesthesia were intercepted, The maxillary and HE were stained with the infiltrating inflammatory cells counting from the gingival connective tissue and epithelial tissue. The distance between the alveolar crest and the enamel cementum was measured. The venous blood was collected from the total abdominal vena cava Serum samples were detected by ELISA method of TNF-α content, using SPSS10.0 software for single-factor analysis of variance. Results: The number of infiltrating inflammatory cells in gingival tissues and the distance from alveolar crest to enamel cementum in group B and group C were higher than those in group A, and the difference between group B and group C was more than that in group C (P <0.05). The content of TNF-α in group B was higher than that in group A and C (P <0.05), but there was no significant difference between group A and group C (P> 0.05). Conclusion: The compound prescription of clearing away heat, nourishing Yin and activating blood can cure the diabetic periodontitis and reduce the level of TNF-α in peripheral blood.