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采用反相高效液相色谱法 ,在流动相 :甲醇 +水 =60 +40 (V/V) ,UV - 2 2 3nm条件下 ,用外标法测定 4 ,4 二甲基 3 异口恶唑酮的含量。该法测定的标准偏差为 0 0 5,变异系数为 0 38% ,回收率为 99 2 4 %～ 10 0 .2 8%。
By reversed - phase high performance liquid chromatography with external standard method, mobile phase: methanol + water = 60 +40 (V / V) Ketone content. The standard deviation of the method was 0 0 5, the coefficient of variation was 0 38% and the recovery rate was 99 2 4% -10 0.