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在诱导子添加的基础上研究了氯化氯代胆碱 (CCC)和肉桂酸 (CA)的浓度和时间因素对红豆杉悬浮培养细胞活力、三尖杉宁碱 (Cephlomannine)和紫杉醇 (Taxol)生物合成的影响 ,并进一步探讨了两者与前体的协同作用。实验表明 :(1)氯化氯代胆碱、肉桂酸对细胞活力的影响均为 :延滞期 >稳定期>对数期 ;(2 ) d18加入氯化氯代胆碱 10 mg/ L ,肉桂酸 2 0 m g/ L可使紫杉醇含量分别提高 10 ,14倍 ;d18加入氯化氯代胆碱 2 0 mg/ L可使三尖杉宁碱含量提高 2倍 ,d12加入肉桂酸 2 0 mg/ L可提高 10倍 ;(3) 4 0 mg/ L氯化氯代胆碱 ,2 0 m g/ L肉桂酸 ,5 m g/ L苯异丝氨酸 ,40 m g/ L甲瓦龙酸 (MVA)内酯对提高紫杉醇产量较优 ;而 10 m g/ L氯化氯代胆碱 ,2 0 m g/ L肉桂酸 ,10 mg/ L苯异丝氨酸 ,2 0 mg/ L甲瓦龙酸 (MVA)内酯对三尖杉宁碱合成有利。
The effects of concentration and time of chlorinated choline chloride (CCC) and cinnamic acid (CA) on cell viability, Cephlomannine and Taxol in suspension culture of Taxus chinensis were studied on the basis of inducer addition. Biosynthesis, and to further explore the synergy between the two and precursors. The experimental results showed that: (1) The effects of chlorinated choline chloride and cinnamic acid on cell viability were as follows: lag phase> stable phase> logarithmic phase; (2) choline chloride 10 mg / L, The content of paclitaxel increased 10- and 14-fold with 20 mg / L and 20 mg / L choline chloride, respectively, while the content of cephalomannine increased by 2 folds. When cinnamic acid 20 mg / L can be increased by 10 times; (3) 4 0 mg / L choline chloride, 20 mg / L cinnamic acid, 5 mg / L benzene isoserine, 40 mg / L MVA lactone (10 mg / L choline chloride, 20 mg / L cinnamic acid, 10 mg / L benzoyl isoserine and 20 mg / L MVA lactone) Cephalomannine synthesis is beneficial.