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[目的]探讨Hg和Cd的遗传毒性。[方法]以赤子爱胜蚓为试验材料,采用滤膜法探讨Hg和Cd单一污染对蚯蚓血细胞微核的影响。[结果]在一定浓度范围内,Hg和Cd单一污染对蚯蚓血细胞微核的产生均有显著影响。微核率随处理浓度的增加呈现先上升后下降的趋势。回归分析显示,Hg在0~25.5 mg/L的浓度范围内,微核率与处理浓度呈极显著正相关(r=0.93,F=224.118,P<0.01),在25.5~63.75 mg/L浓度范围内,微核率与处理浓度呈极显著负相关(r=0.85,F=127.255,P<0.01)。Cd在0~115 mg/L浓度范围内,微核率与处理浓度呈极显著正相关(r=0.69,F=63.667,P<0.01),在115~143.75 mg/L浓度范围内,微核率与处理浓度呈极显著负相关(r=0.94,F=137.413,P<0.01)。[结论]该研究提供了一种新的检测土壤或水中重金属污染的生物学方法。
[Objective] To investigate the genotoxicity of Hg and Cd. [Method] With Eisenia foetida as test material, the effect of Hg and Cd single pollution on the micronuclei of earthworm blood cells was studied by using the filter membrane method. [Result] The single pollution of Hg and Cd had a significant effect on the production of micronuclei in earthworms in a certain concentration range. The micronucleus rate increased first and then decreased with the increase of treatment concentration. Regression analysis showed that there was a significant positive correlation between the micronucleus rate and the treatment concentration (r = 0.93, F = 224.118, P <0.01) for Hg at the concentration range of 0-25.5 mg / L, and at a concentration of 25.5-63.75 mg / Within the range, the micronucleus rate was significantly and negatively correlated with the treatment concentration (r = 0.85, F = 127.255, P <0.01). In the concentration range of 0 ~ 115 mg / L, there was a significant positive correlation between the micronucleus rate and the treatment concentration of Cd (r = 0.69, F = 63.667, P <0.01) There was a significant negative correlation between the rate and the treatment concentration (r = 0.94, F = 137.413, P <0.01). [Conclusion] The study provided a new biological method to detect heavy metal pollution in soil or water.