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对三种方法检测戊二醛溶液、二氯异氰尿酸钠溶液杀灭枯草杆菌黑色变种芽孢的效果进行了比较。结果,用欧洲标准悬液定量杀菌试验.改良欧洲悬液定量杀菌试验和载体浸泡定量杀菌试验检测含戊二醛2％（g/ml）溶液杀灭枯草杆菌黑色变种芽孢达消毒合格标准,作用时间分别为2.5、3.0、2.0h。用前两方法检测含有效氯4500mg/L的二氯异氰尿酸钠溶液及用载体方法检测含有效氯1000mg/L该液杀灭枯草杆菌黑色变种芽孢达消毒合格标准,作用时间分别为30、20、10min、当染菌片的芽孢悬液中增含小牛血清50％（ml/ml）时,用载体方法检测含有效氯5000mg/L的二氯异氰尿酸钠溶液杀灭该芽孢达消毒合格标准,作用时间近20min,与用欧洲标准悬液法检测者（30min）相近。表明三种方法检测受有机物影响较小的戊二醛杀菌效果相差较小,检测受有机物影响较大的二氯异氰尿酸钠杀菌效果相差较大。后者可能与方法中含有机物多少有关。
The effects of three methods on detecting glutaraldehyde solution and sodium dichloroisocyanurate solution to kill B. subtilis black mutant were compared. Results, the European standard suspension quantitative disinfection test improved quantitative disinfection of the European suspension test and carrier immersion quantitative bactericidal test detection of glutaraldehyde 2% (g / ml) solution to kill B. subtilis black spores up to disinfection standards, the role of Time was 2.5,3.0,2.0 h. Using the first two methods to detect containing sodium chloride 4500mg / L sodium dichloroisocyanurate solution and the carrier method containing chlorine containing 1000mg / L of the liquid to kill B. subtilis black mutant spores up to disinfection standards, the action time was 30, 20, 10min, when the bacterial suspension of the bacterial suspension contains 50% (ml / ml) of calf serum, the carrier method is used to detect the sodium dichloroisocyanurate solution containing available chlorine 5000mg / L to kill the spore reached Disinfection pass standards, the role of time nearly 20min, and the European standard suspension test (30min) similar. The results showed that there was a little difference between the three methods in detecting the bactericidal effect of glutaraldehyde which is less affected by organic matter. The difference of the bactericidal effect of dichloroisocyanurate detected by the organics was quite different. The latter may be related to how much organic matter is contained in the method.