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[Objective] This study aimed to compare the effects of different processing methods on the content of chlorogenic acid in Fructus Gardeniae. [Method] The chromatographic separation was performed on an Inersil ODS-2 C18column(250 mm × 4. 6 mm,5 μm) with acetonitrile- 0. 1% acetic acid solution(12∶ 88,V/V) as the mobile phase under conditions of flow rate 1. 0 ml/min,detection wavelength 327 nm,column temperature 25 ℃. [Result]With a range of0. 42- 2. 52 μg / μl,chlorogenic acid concentration exhibited a good linear relationship with peak area( n = 6,R = 0. 999 9). The average recovery rate was100. 83%,RSD was 1. 30%. [Conclusion] The established method is convenient,rapid and accurate with good repeatability,which can be used to effectively control the quality of different processed products of Fructus Gardeniae.
[Objective] This study aimed to compare the effects of different processing methods on the content of chlorogenic acid in Fructus Gardeniae. [Method] The chromatographic separation was performed on an Inersil ODS-2 C18column (250 mm × 4.6 mm, 5 μm ) with acetonitrile- 0.1% acetic acid solution (12:88, V / V) as the mobile phase under conditions of flow rate 1.0 ml / min, detection wavelength 327 nm, column temperature 25 ° C. [Result] With a range of 0. 42- 2. 52 μg / μl, chlorogenic acid concentration showed a good linear relationship with peak area (n = 6, R = 0.9999). The average recovery rate was100. 30%. [Conclusion] The established method is convenient, rapid and accurate with good repeatability, which can be used effectively to the quality of different processed products of Fructus Gardeniae.