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目的观察脂质体介导的VEGF质粒对成年大鼠脑缺血再灌注后大脑神经元phosphorylation of methyl-CpG binding protein 2 (P-MeCP2)表达的影响,探讨VEGF促进缺血损伤后神经元新生的可能机制,进一步为VEGF治疗缺血性脑中风提供实验和理论依据。方法 30只SD大鼠,随机分为假手术组(sham组)、质粒组(VEGF组)和对照质粒组(vehicle组),采用左侧大脑中动脉线栓(MCAO)模型,侧脑室给药,免疫印迹、免疫荧光三标染色及激光共聚焦扫描技术等方法检测P-MeCP2的表达。结果再灌2周VEGF质粒组P-MeCP2、BrdU和NeuN同时表达于缺血侧大脑皮质神经元,而缺血对侧没有找到,VEGF质粒组皮质内P-MeCP2的表达较对照组、假手术组显著增高(p<0.01)。结论脂质体介导的VEGF质粒能促进成年大鼠脑缺血神经元的新生,可能与上调P-MeCP2的表达有关。
Objective To observe the effect of liposome-mediated VEGF plasmid on the expression of phosphorylation of methyl-CpG binding protein 2 (P-MeCP2) in adult rat brain after cerebral ischemia-reperfusion Which may provide experimental and theoretical basis for VEGF treatment of ischemic stroke. Methods Thirty SD rats were randomly divided into sham group, VEGF group and control vehicle group. The left middle cerebral artery occlusion (MCAO) model and lateral ventricle administration , Western blotting, immunofluorescence triple staining and confocal laser scanning technique were used to detect the expression of P-MeCP2. Results P-MeCP2, BrdU and NeuN in VEGF plasmid group were both expressed in ischemic cortex neurons at 2 weeks after reperfusion, but not found in ischemic contralateral side. The expression of P-MeCP2 in cortex of VEGF plasmid group was significantly lower than that in control group Group was significantly higher (p <0.01). Conclusion The liposome-mediated VEGF plasmid can promote the regeneration of cerebral ischemia neurons in adult rats, which may be related to the up-regulation of the expression of P-MeCP2.