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灵芝是我国著名传统药用真菌,假芝属(Amauroderma)真菌作为灵芝科重要属,我国已报道种共有22个.本研究从广州白云山采集到一株野生假芝子实体,经组织分离获得菌丝体纯培养,编号GZ-01菌株.利用形态鉴定和ITS鉴定手段,确定其分类学地位为假芝属,系统命名为A.subrugosum,为中国首次记录种,菌种保藏号ACCC52954.生物学特性研究表明,菌丝生长的最适碳源为甘露醇,最适氮源为酵母浸膏或大豆蛋白胨,最适C/N比为20/1,最适生长因子为维生素B6,最适生长温度为28℃,最适p H值范围为6.0-7.5.本研究获得新的药用真菌资源,其培养条件研究可为进一步开发利用奠定实验基础.
Ganoderma lucidum is a famous traditional medicinal fungus in our country, and the fungi of Amauroderma are the important genera of Ganoderma lucidum, and there are 22 species reported in China.In this study, a wild pseudorasome fruiting body was collected from Baiyun Mountain, Guangzhou, The mycelia were cultured in pure culture with GZ-01 strain.Using morphological identification and ITS identification, the taxonomic status of the mycelium was identified as Pseudomonas, the system was named A.subrugosum, which was recorded for the first time in China, and the strain’s accession number was ACCC52954. Studies on the characteristics of mycelium showed that the optimum carbon source for mycelial growth was mannitol, the optimum nitrogen source was yeast extract or soy peptone, the optimum C / N ratio was 20/1, the optimum growth factor was vitamin B6, The growth temperature was 28 ℃, and the optimum p H value ranged from 6.0 to 7.5. The new medicinal fungi resources were obtained in this study, and the study of its culture conditions could lay the experimental foundation for further development and utilization.