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本文采用Gupta等介绍的EAγ花环形成法和酯酶染色双标记的方法对正常人外周血淋巴细胞ANAE颗粒类型与细胞表面标志的关系进行了初步探索。结果表明,用E花环分离的淋巴细胞(T细胞)中99.6％为ANAE阳性,EAγ阳性的T细胞中77％在ANAE染色上呈“分散颗粒型”,所以,“分散颗粒型”T细胞可以被认为主要代表Tr细胞。但是,EAγ~+细胞仍有23％呈“斑状颗粒型”,EAγ~-细胞也有8.3％呈“分散颗粒型”。因此,著者认为MAγ~+细胞和“分散颗粒型”细胞可能都不是均质的群体。
In this paper, Gupta et al introduced the method of EA gamma rosette formation and esterase staining double labeling of normal peripheral blood lymphocytes ANAE particle type and cell surface markers were explored. The results showed that 99.6% of lymphocytes (T cells) isolated from the E rosette were ANAE-positive, and 77% of the EAγ-positive T cells were “scattered particle type” on the ANAE staining. Therefore, “dispersed granular T cells” Trichoderma is thought to represent predominantly Tr cells. However, 23% of EAγ ~ + cells showed “patchy granule” and 8.3% of EAγ ~ - cells showed “scattered granule”. Therefore, the authors consider that both MAy ~ + cells and “dispersed particle type” cells may not be homogeneous populations.