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安徽省明光市 2 0 0 1年 6～ 7月发生了一起无菌性脑膜脑炎流行 ,从病人脑脊液和粪便标本中分离到 17株柯萨奇 (Coxsackie ,Cox .)B5病毒 ,血清学实验证明所分离的病毒为此次无菌性脑膜脑炎流行的病因。对其中的 2株病毒 (MG/ 18/ 2 0 0 1和MG/ 39/ 2 0 0 1)测定了VP1区全基因核酸序列并做了比较分析。结果显示 :所测 2株病毒VP1区核苷酸长度均为 849bp ,两毒株之间仅有 1个核苷酸不同。这两株病毒与从 (GenBank)检索到的Cox B5病毒的核苷酸同源性约为 82 %,氨基酸序列亦有改变。从进化树上看 ,这两株病毒同在一个小分支上 ,与它们亲缘关系最近的是从一疑似脊髓灰质炎的儿童分离到的AF114383 -Cox B5病毒 ,而与从手足口病患者分离到的X6 770 6-Cox B5病毒相对较远。表明此次脑膜脑炎的病因病毒与同是侵犯中枢神经系统的Cox B5的原型株 -AF114383 -Cox B5病毒亲缘关系最近 ,但核苷酸已经发生了 >18%的变异 ,可以认为其是Cox B5新亚型病毒。
Aseptic meningoencephalitis occurred in June-July 2001 in Mingguang City, Anhui Province, and 17 Coxsackie (Cox.) B5 viruses were isolated from cerebrospinal fluid and stool specimens of patients. Serological tests Proof of the virus isolated for the aseptic meningoencephalitis epidemic etiology. The two strains of viruses (MG / 18/2001 and MG / 39/2001) were sequenced and compared with the VP1 region. The results showed that the nucleotide length of VP1 region of the two viruses tested was 849bp, and only one nucleotide differed between the two strains. The two viruses share about 82% nucleotide homology with the Cox B5 virus retrieved from GenBank, and the amino acid sequence is also changed. From the phylogenetic tree, the two viruses are on the same small branch, the closest of which is the AF114383-Cox B5 virus isolated from a child suspected of having poliomyelitis, but not isolated from HFD patients X6 770 6-Cox B5 virus is relatively far away. The causative virus of meningoencephalitis shows the closest relationship with the Cox B5 prototypic strain-AF114383-Cox B5 virus, which is the same type of central nervous system. However, the nucleotide has mutated 18%, which is considered as Cox B5 new subtype virus.