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目的:探讨人类冠状动脉粥样硬化病变类型与内膜α-平滑肌肌动蛋白(α-SMA)含量的关系。方法:选用53例尸检病例的312块冠状动脉组织。经苏木精-伊红染色诊断冠状动脉粥样硬化病变及其类型,用免疫组化和Scion图像软件系统,测定和计算冠状动脉标本中α-SMA阳性面积%。结果:在冠状动脉粥样病变类型中,5%(16/312)为Ⅰ型,10%(31/312)为Ⅱ型,21%(66/312)为Ⅲ型,4%(14/312)为Ⅳ型,18%(55/312)为Ⅴ型和2%(6/312)为Ⅵ型。冠状动脉粥样硬化病变内膜α-SMA阳性面积%随着病变进展和管腔狭窄程度的加重而减少,分别呈负相关(r=-0.494,P<0.01,r=-0.362,P<0.01)。结论:人类冠状动脉粥样硬化病变内膜α-SMA阳性面积%随着冠状动脉粥样硬化病变程度的加重而减少。
Objective: To investigate the relationship between the type of coronary atherosclerosis and the content of α-smooth muscle actin (α-SMA) in human. Methods: A total of 312 coronary arteries from 53 autopsy cases were selected. The lesions and types of coronary atherosclerosis were detected by hematoxylin-eosin staining. The positive area of α-SMA in coronary samples was determined and calculated by immunohistochemistry and Scion software. Results: Among the types of coronary atherosclerosis, 5% (16/312) were type Ⅰ, 10% (31/312) were type Ⅱ, 21% (66/312) were type Ⅲ and 4% ) Is type IV, 18% (55/312) is type Ⅴ and 2% (6/312) is type Ⅵ. Coronary atherosclerotic lesion endometrial α-SMA-positive area% with the progression of the disease and the degree of stenosis decreased, respectively, were negatively correlated (r = -0.494, P <0.01, r = -0.362, P ). Conclusions: The percentage of α-SMA positive area in intima of human coronary atherosclerotic lesion decreases with the severity of coronary atherosclerosis.