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目的:探讨成纤维细胞生长因子-10(FGF10)及其受体(FGFR2b 或 Bek)在胎儿皮肤附件(SA)形成中的表达特征及其生物学意义。方法:对130块分别来自26例不同胎龄(EGA 8～31周)胎儿,5个不同部位(头、下颌、耳垂、肩和胸骨柄)的皮肤标本,采用病理学、免疫组化方法和计算机图像扫描技术检测 FGF10、Bek、细胞角蛋白-19(CKl9)、Bcl-2和细胞增殖核抗原(PCNA)在胎儿皮肤组织的表达水平。结果:除 EGA8周胎儿缺乏典型的皮肤组织学结构和 FGF10和 Bek 蛋白表达外,所有蛋白在 E11周以后的皮肤内都有阳性表达。在EGA11周,表皮下成团分布的间质细胞过表达 FGF10、PCNA 和 Bcl-2,表皮细胞和周皮细胞则所有蛋白均呈强阳性表达;在 EGA13周时表皮细胞局灶性增殖形成 SA 胚芽,并逐渐向真皮内生长、分化和迁移;在 EGA11～31周的皮肤组织内,真皮内问质细胞表达 FGF10、PCNA 和 Bcl-2以及 SA 上皮细胞表达 FGF10、Bek、PCNA、CK19和 Bcl-2蛋白呈现与生长发育同步的表达特征,但单个细胞表达的平均光密度(A)值则随胎龄增加而逐渐下降(P<0.05～0.001)。结论:间质细胞旁分泌的 FGF10与上皮细胞膜 Bek 特异性结合,是诱导胚胎 SA 上皮细胞生长及其形态发生的重要信号,但有关 SA 的形成部位和种类及其与 FGF10蛋白表达的相互关系仍有待深入研究。
Objective: To investigate the expression of fibroblast growth factor-10 (FGF10) and its receptor (FGFR2b or Bek) in fetal skin attachment (SA) formation and its biological significance. Methods: Totally 130 skin specimens from 26 fetuses with different gestational ages (EGA 8-31 weeks), 5 different parts (head, mandible, earlobe, shoulder and sternum) from 26 fetuses of different gestational ages (EGA 8-31 weeks) were examined by pathology and immunohistochemistry The expression of FGF10, Bek, cytokeratin-19 (CKl9), Bcl-2 and proliferating cell nuclear antigen (PCNA) in fetal skin tissues were detected by computer image scanning. RESULTS: All of the proteins were positive in the skin after E11 weeks, except for the absence of the typical dermal histology and the expression of FGF10 and Bek proteins. In the EGA11 weeks, the interstitial cells distributed in interstitial cells over-expressing FGF10, PCNA and Bcl-2, epidermal cells and pericytes all showed strong positive expression of all proteins; EGA 13 weeks epidermal cells proliferate to form SA Germ, and gradually to the dermis growth, differentiation and migration; within 11 to 31 weeks of skin EAG, intraepidermal cells express FGF10, PCNA and Bcl-2 and SA epithelial cells express FGF10, Bek, PCNA, CK19 and Bcl -2 protein showed synchronous expression of growth and development, but the average optical density (A) value of single cells decreased with the increase of gestational age (P <0.05 ~ 0.001). CONCLUSIONS: The specific binding of stromal cell-paracrine FGF10 to the epithelial cell membrane Bek is an important signal to induce the growth and morphogenesis of embryonic SA. However, the location and type of SA and its relationship with FGF10 protein expression To be further studied.