目的:探讨不同方法提取化瘀消癥复方(丹参、桃仁、赤芍、天花粉和紫草)对人源妊娠滋养细胞系HTR-8/SVneo细胞凋亡的影响。方法:采用CCK8法检测经不同方法提取的化瘀消癥复方(A水提、B醇提、C醇提后药渣水提、D醇提+水提)对HTR-8/SVneo细胞增殖的影响;流式细胞术检测48 h后细胞凋亡情况;设甲氨蝶呤组为阳性对照组,未经药物处理的细胞为阴性对照组。结果:不同提取方法的中药复方对HTR-8/SVneo细胞作用后对其增殖均有抑制作用,其中A组抑制率大于B、C、D组(P<0.05);其凋亡率分别为(24.37±3.24)%(A组),(12.83±0.37)%(B组),(11.70±0.48)%(C组),(14.37±0.51)%(D组),(18.05±1.27)%(MTX组),与阴性对照组(7.057±0.76)%相比,各组凋亡率均明显增加(P<0.01);其中A组凋亡率高于MTX组、B组、C组、D组(P<0.01)。结论:不同提取方法提取化瘀消癥复方对HTR-8/SVneo细胞凋亡的影响,以水提组最明显,可将水提法作为化瘀消癥复方后续研究的中药提取方法。 OBJECTIVE: To investigate the effects of different methods on the apoptosis of human gestational trophoblast cell line HTR-8 / SVneo induced by Huayu Xiaozheng Compound (Salvia miltiorrhiza, peach kernel, Radix Paeoniae Alba, trichosanthin and Arnebia euchroma). Methods: CCK8 assay was used to detect the proliferation of HTR-8 / SVneo cells induced by different methods of extraction of blood stasis and eliminating blood stasis compound A (A water extract, alcohol B extract, water extract of C alcohol after extraction, alcohol extract + water extract) The cell apoptosis was detected by flow cytometry after 48 h. Methotrexate group was positive control group, and the untreated cells were negative control group. Results: The inhibitory rates of HT-8 / SVneo-treated HTR-8 / SVneo cells were significantly lower than those of B, C and D groups (P <0.05). The apoptotic rates were 24.37 ± 3.24% in group A, 12.83 ± 0.37% in group B, 11.70 ± 0.48% in group C, 14.37 ± 0.51% in group D, and 18.05 ± 1.27% in group D MTX group). Compared with the negative control group (7.057 ± 0.76)%, the apoptosis rate in each group was significantly increased (P <0.01). The apoptosis rate in group A was higher than that in MTX group, B group, C group and D group (P <0.01). Conclusion: The effects of different extraction methods on the apoptosis of HTR-8 / SVneo cells induced by Huayu Xiaozhang were the most obvious. The water extraction could be used as the traditional Chinese medicine extraction method for the follow-up study of Huayu Xiaozhong Compound.