目的探讨银杏叶总黄酮体外对人肝癌细胞HepG2增殖与凋亡的影响。方法将银杏叶总黄酮作用于体外培养的人肝癌细胞HepG2,MTT法检测药物对HepG2细胞增殖的影响,缺口末端核苷标记(TUNEL)法检测药物对HepG2细胞凋亡的影响。结果银杏叶总黄酮对体外培养的人肝癌细胞HepG2的增殖效率下降,使凋亡细胞数增加(P<0.01),呈剂量依赖效应。结论银杏叶总黄酮对体外培养的人HepG2细胞增殖有抑制作用,并能诱导细胞凋亡。 Objective To investigate the effects of total flavonoids of Ginkgo biloba leaves on proliferation and apoptosis of HepG2 human hepatocellular carcinoma cells in vitro. Methods The total flavonoids of Ginkgo biloba were treated with HepG2 cells in vitro. MTT assay was used to detect the effect of HepG2 cells on the proliferation of HepG2 cells. TUNEL method was used to detect the effect of HepG2 cells on apoptosis. Results The total flavonoids of Ginkgo biloba decreased the proliferation of HepG2 cultured in vitro and increased the number of apoptotic cells (P <0.01) in a dose-dependent manner. Conclusion The total flavonoids of Ginkgo biloba can inhibit the proliferation of human HepG2 cells cultured in vitro and induce cell apoptosis.