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目的分析Mylpf基因在糖尿病长爪沙鼠6种组织中的表达水平,进而探究长爪沙鼠糖尿病发生的分子机制。方法选取糖尿病长爪沙鼠和正常沙鼠各6只,使用Real-time PCR和Western blotting技术分别检测动物的骨骼肌、肝脏、脂肪、肾脏、心脏和脑组织中Mylpf的mRNA和蛋白表达水平。结果 Real-time PCR的结果表明,在骨骼肌、心脏和脑组织中,Mylpf在糖尿病组mRNA表达水平高于对照组。在脂肪组织和肝脏组织中糖尿病组mRNA表达水平低于对照组。在肾脏组织中两组mRNA表达水平基本相同。Western blotting结果显示,在骨骼肌中Mylpf在糖尿病组蛋白表达水平高于对照组,且有统计学差异,在心脏和脂肪组织中Mylpf在糖尿病组蛋白表达水平低于对照组,在肾脏组织中蛋白表达水平基本相同,在肝脏和脑组织中没有检测出。结论 Mylpf与长爪沙鼠糖尿病的发生有一定相关性,该基因对长爪沙鼠糖尿病的影响可能主要发生在骨骼肌和心脏组织中。
Objective To analyze the expression of Mylpf gene in 6 tissues of Mongolian gerbil (Diabetes mellitus), and to explore the molecular mechanism of Mylpf gene in diabetic gerbils. Methods Six diabetic gerbils and normal gerbils were selected and their Mylpf mRNA and protein expression levels in skeletal muscle, liver, fat, kidney, heart and brain were detected by Real-time PCR and Western blotting respectively. Results The result of Real-time PCR showed that Mylpf mRNA expression in skeletal muscle, heart and brain was higher than that in the control group. In adipose tissue and liver tissue, mRNA expression in diabetic group was lower than that in control group. In the kidney tissue two groups of mRNA expression levels are basically the same. Western blotting results showed that Mylpf in skeletal muscle in diabetic group protein expression levels higher than the control group, and there was a statistically significant difference in heart and adipose tissue Mylpf in diabetic group protein expression levels in the control group, in the kidney tissue protein The expression levels are basically the same, not detected in the liver and brain tissue. Conclusion Mylpf may be related to the development of diabetes in Mongolian gerbils. The effects of this gene on the diabetes mellitus in gerbils may mainly occur in skeletal muscle and heart tissues.