论文部分内容阅读
目的建立肿瘤Ⅰ号胶囊中丹参素与淫羊藿苷的含量测定方法。方法采用HPLC法,以乙腈-体积分数为1%的冰醋酸溶液梯度洗脱,色谱柱为Kromasil C18(4.6 mm×200 mm,5μm),流速为1.0 mL.min-1,柱温为35℃,检测波长为275 nm。结果丹参素在11.38~227.50 mg.L-1内,淫羊藿苷在2.0~40.0 mg.L-1内,与峰面积呈良好的线性关系,相关系数分别为0.9995和0.9996。方法平均回收率分别为98.6%(RSD=1.9%)、96.0%(RSD=0.84%)。结论可作为肿瘤Ⅰ号胶囊质量控制方法之一。
Objective To establish a method for the determination of Danshensu and icariin in Tumor No.1 Capsule. Methods The HPLC method was used. The gradient elution was acetonitrile-1% glacial acetic acid. The column was Kromasil C18 (4.6 mm×200 mm, 5 μm), the flow rate was 1.0 mL.min-1, and the column temperature was 35°C. The detection wavelength is 275 nm. RESULTS: Within the range of 11.38-227.50 mg.L-1 for danshensu and within 2.0-40.0 mg.L-1 for icariin, there was a good linear relationship with peak area. The correlation coefficients were 0.9995 and 0.9996, respectively. The average recovery of the method was 98.6% (RSD=1.9%) and 96.0% (RSD=0.84%), respectively. The conclusion can be used as one of the quality control methods for tumor I capsules.