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目的探讨益气养阴散结方含药血清对人肺癌A549细胞增殖与凋亡的影响。方法应用MTT比色法检测益气养阴散结方对肺癌细胞增殖的抑制率,并用AnnexinV/PI染色法检测凋亡率。结果药物组肺癌A549细胞的增殖明显弱于对照组(P<0.05),凋亡率明显高于对照组(P<0.05)。结论益气养阴散结方含药血清能明显抑制A549细胞的增殖,并诱导其凋亡。
Objective To investigate the effects of Yiqi Yangyin Sanjie Prescription on proliferation and apoptosis of human lung cancer A549 cells. Methods MTT colorimetric method was used to detect the inhibitory rate of proliferation of lung cancer cells by using Yiqi Yangyin Sanjie Decoction. The apoptosis rate was detected by Annexin V / PI staining. Results The proliferation of lung cancer A549 cells in the drug group was significantly weaker than that in the control group (P <0.05), and the apoptosis rate was significantly higher in the drug group than in the control group (P <0.05). Conclusion Yiqi Yangyin Sanjie Prescription containing serum can significantly inhibit the proliferation of A549 cells and induce its apoptosis.