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比较冷冻和化学固定后骨骼肌超微结构的不同特征。方法 :蟾蜍缝匠肌经常规化学固定以及超低温快速冷冻固定、冷冻置换后 ,透射电镜进行超微结构观察。结果 :冷冻固定后 ,缝匠肌基膜仅仅由电子密度高的一层组成 ;横小管为圆形 ;终池内含有电子密度高的环形物 ,终池膜上存在一排线状电子密度高的蛋白颗粒。化学固定后 ,缝匠肌基膜由两层组成 :一层电子密度低 ,另一层电子密度高 ;横小管为扁平状或哑铃状 ;终池内仅有一些散在的电子密度高的颗粒 ,终池膜上有几个脚状突起伸向横小管。结论 :冷冻和化学固定后 ,骨骼肌基膜、横小管、终池等结构存在着不同的形态特点。这些不同的形态形成的原因及生理意义还有待进一步研究。
Different characteristics of skeletal muscle ultrastructure after freezing and chemical fixation were compared. Methods: The toad sartorius muscle was routinely chemically fixed and cryopreserved by cryopreservation. After cryopreservation, ultrastructure was observed by transmission electron microscopy. Results: After frozen fixation, the sartorius muscle myometrium consisted of only one layer with high electron density; the transverse tube was round; the final cell contained a ring of electron density and a row of linear electron density on the final cell membrane Protein particles. After chemical fixation, the sartorius muscle basement membrane consists of two layers: one with low electron density and the other with high electron density; the transverse tubule is flat or dumbbell-shaped; there are only a few scattered electronically dense particles in the final cell There are several projections on the diaphragm extending to the lateral tubules. Conclusion: There are different morphological characteristics of skeletal muscle basement membrane, transverse tubule and terminal pool after frozen and chemically fixed. The causes of formation of these different morphological and physiological significance needs further study.