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分析能引起红辣椒变色斑块的细菌和真菌侵染后果肉组织中丙二醛含量、细胞膜相对透性及细胞微观结构的变化,结果显示,“花皮”致病菌侵染后,果肉组织丙二醛含量、细胞膜相对透性较对照明显增加(P<0.05),导致细胞结构、细胞内有色体结构破坏,色素组分分解,其中致病细菌主要是对果肉组织的外层与内层细胞同时破坏,而致病真菌主要从果实内层向外层破坏细胞结构,且对细胞壁破坏作用较细菌强,更容易破坏有色体结构。不同致病菌对辣椒组织破坏能力存在差异,枯草芽孢杆菌(Bacillus subtilis)、白腐菌(Phanerochaete sordida)和白囊耙齿菌(Irpex lacteus)对细胞壁、细胞膜及有色体结构破坏较球形赖氨酸芽孢杆菌(Lysinibacillus sphaericus)、枝状枝孢菌(Cladosporium cladosporioides)严重,使大部分内含物及类胡萝卜素分解,剩下少许脂滴。试验结果表明,“花皮”致病菌侵染辣椒严重破坏其组织细胞结构并导致细胞有色体破坏,色素分解,从而引起外观色泽褪变。
The changes of malondialdehyde (MDA) content, relative permeability of cell membrane and cell microstructure in flesh and fungus infected by bacteria and fungi which caused chromatic discoloration of red pepper were analyzed. The results showed that after infection with “Huapi” The content of malondialdehyde (MDA) and the relative permeability of cell membrane in the pulp tissue were significantly increased compared with the control (P <0.05), which led to the destruction of the cell structure, the destruction of intracellular chromosomal structures and the breakdown of pigment components. Pathogenic bacteria mainly affected the outer layer of pulp tissue While the inner layer of cells destroyed at the same time, while the pathogenic fungi mainly destroy the cell structure from the inner layer of fruit to the outer layer, and the destruction of the cell wall is stronger than the bacteria, more likely to destroy the chromosomal structure. Different pathogenic bacteria have different ability of destroying pepper tissue. Bacillus subtilis, Phanerochaete sordida and Irpex lacteus have more damage to cell wall, cell membrane and color body than spherical lysine Lysinibacillus sphaericus, Cladosporium cladosporioides are severe, causing most of the contents and carotenoids to disintegrate leaving a small amount of lipid droplets. The experimental results showed that the infection of peppers by the “Huapi” germs severely damaged the cell structure of the cells and led to the destruction of the chromosomal cells, the breakdown of the pigments, and the fading of the appearance color.