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目的:观察皮质酮(CORT)对培养的脊髓背角神经元Ca2+浓度([Ca2+]i)的调节作用及机制。方法:培养新生SD大鼠脊髓背角神经元,激光共聚焦显微镜检测神经元[Ca2+]i的变化。结果:CORT可快速升高培养的脊髓背角神经元[Ca2+]i,且呈现剂量依赖性(P<0.05);CORT诱导的神经元[Ca2+]i升高是以外钙内流为主(P<0.01);百日咳毒素(G蛋白活化阻断剂)可阻断CORT所致的脊髓背角神经元[Ca2+]i升高(P<0.01),而糖皮质激素受体拮抗剂RU38486对CORT的效应无抑制作用。结论:CORT通过非基因组途径快速增高培养的脊髓背角神经元[Ca2+]i。
AIM: To investigate the regulatory effect of cortisone (CORT) on Ca2 + concentration ([Ca2 +] i) in cultured spinal dorsal horn neurons and its mechanism. Methods: Spinal dorsal horn neurons were cultured in neonatal SD rats. The changes of [Ca2 +] i in neurons were detected by laser confocal microscopy. RESULTS: CORT increased the [Ca2 +] i of spinal dorsal horn neurons in a dose-dependent manner (P <0.05). CORT-induced elevation of [Ca2 +] i was mainly due to extracellular calcium influx <0.01). Pertussis toxin (a G-protein activator blocker) could block the increase of [Ca2 +] i in spinal dorsal horn neurons caused by CORT (P <0.01), while the effect of glucocorticoid receptor antagonist RU38486 on CORT No effect of inhibition. Conclusion: CORT rapidly increases [Ca2 +] i in spinal dorsal horn neurons by non-genomic pathway.