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目的观察联用乳酸杆菌和丁酸梭菌对小鼠溃疡性结肠炎(UC)的疗效并探讨其治疗机制。方法建立葡聚糖硫酸钠(Dextran sulfate Sodium,DSS)诱导的小鼠急性UC模型,观察给予乳酸杆菌和丁酸梭菌治疗后,小鼠结肠粘膜的病理改变和肿瘤坏死因子(TNF-α)和单核细胞趋化蛋白-1(MCP-1)表达的变化。结果乳酸杆菌和丁酸梭菌可明显减轻小鼠结肠粘膜的损伤;可明显抑制TNF-α和MCP-1的表达,尤以两菌合用组抑制作用最强。结论乳酸杆菌和丁酸梭菌对DSS诱导的UC有治疗作用,对TNF-α表达的协同抑制作用可能是其发挥协调治疗作用的分子机制。
Objective To observe the therapeutic effect of Lactobacillus and Clostridium butyricum on murine ulcerative colitis (UC) and to explore its therapeutic mechanism. Methods The acute UC model induced by dextran sulfate sodium (DSS) was established. The pathological changes of colonic mucosa and tumor necrosis factor (TNF-α) in mice were observed after treatment with Lactobacillus and Clostridium butyricum And monocyte chemotactic protein-1 (MCP-1) expression changes. Results Lactobacillus and Clostridium butyricum could obviously reduce the damage of colonic mucosa in mice and significantly inhibit the expression of TNF-α and MCP-1, especially in combination with two bacteria. Conclusion Lactobacilli and Clostridium butyricum have a therapeutic effect on DSS-induced UC, and the synergistic inhibitory effect on TNF-α expression may be the molecular mechanism of coordination therapy.