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目的探讨核桃补充饮食对发育期小鼠认知功能的影响。方法初断乳昆明小鼠60只,体质量11~14g,分为:对照(常规饲料)、3%核桃和6%核桃(在常规饲料里核桃的配比分别为3%、6%)三组,分别采用旷场箱、避暗箱及八臂迷宫检测小鼠的自发活动、被动逃避能力及空间学习记忆能力,对比研究核桃对小鼠认知功能的改善作用。结果核桃干预对小鼠的摄食行为和体质量无影响。与对照组比,3%、6%核桃干预后小鼠的自主活动能力提高(P<0.05);3%核桃组探索活性增强(P<0.05),6%核桃组探索活性亦有增强趋势。八臂迷宫测试中,三组的总探索时间、参考记忆错误次数和工作记忆错误次数随天数增加均呈下降趋势。两个核桃干预组的参考记忆、工作记忆错误次数明显少于对照组(P<0.05),探索时间明显缩短(P<0.05)。结论核桃干预6w后,可使小鼠的自发活动能力增强、空间学习记忆能力显著提高,提示核桃能够明显改善发育期小鼠的认知功能。
Objective To investigate the effect of walnut diet on the cognitive function in developing mice. METHODS: Sixty Kunming mice were randomly divided into control (normal diet), 3% walnuts and 6% walnuts (3% and 6%, respectively) Groups were used open box, dark box and eight-armed maze test spontaneous activity, passive avoidance ability and spatial learning and memory ability, the comparative study of walnut mice cognitive function improvement. Results There was no effect of walnut intervention on ingestion and body weight of mice. Compared with the control group, 3% and 6% of walnuts could increase the autonomic activity of mice (P <0.05), explore the activity of 3% of walnuts (P <0.05), and explore the activity of 6% of walnuts. In the eight-arm maze test, the total exploration time, the number of reference memory errors and the number of working memory errors of the three groups showed a decreasing trend with the increase of the number of days. The reference memory and working memory errors of the two walnut intervention groups were significantly less than those of the control group (P <0.05), and the exploration time was significantly shortened (P <0.05). Conclusion After 6 weeks of walnut intervention, the spontaneous activity of mice increased and the spatial learning and memory abilities significantly increased, suggesting that walnuts can significantly improve cognitive function in developing mice.