论文部分内容阅读
目的研究轴索损伤标志物神经丝蛋白轻链(NF-L)及其抗体检测对多发性硬化(MS)诊断及预后的意义,并进一步揭示 MS 的发病机制。方法以酶联免疫吸附实验(ELISA)方法检测了63例 MS 患者、15例视神经脊髓炎(ONM)及其他各对照组[31例其他神经系统炎性疾病(OIND)、18例其他非炎性神经系统疾病(NIND)以及46名神经系统正常对照(NC)]血清和脑脊液中 NF-L 及其抗体、抗髓鞘碱性蛋白(MBP)、抗髓鞘少突胶质细胞糖蛋白(MOG)抗体水平。正态分布资料用x~-±s表示,非正态分布资料用 M(QR)表示。结果脑脊液中 NF-L 浓度 MS 组为26(33)ng/L,ONM组为20(92)ng/L,OIND 组为19(82)ng/L,NIND 组为30(194)ng/L,NC 组为10(7)ng/L。其他各组脑脊液中 NF-L 浓度与 NC 组相比均明显增高(均 P<0.01);NF-L 抗体水平 MS 组低于 OIND 组,高于 NIND 组及 NC 组(均 P<0.05)。MS 患者脑脊液中 NF-L 抗体水平与 MBP 抗体水平(r=0.784,P<0.01)和 IgG 水平(r=0.675,P<0.01)显著相关;但与 MOG 抗体水平无相关性。结论 NF-L 或其抗体测定不能作为鉴别各种神经系统疾病的理想标志物;MS、ONM、OIND、NIND 各组中均存在轴索损伤;炎症可能并非是 NF-L 抗体产生从而导致轴索损伤的主要原因。
Objective To investigate the significance of detection of NF-L and its antibody on the diagnosis and prognosis of multiple sclerosis (MS), and further reveal the pathogenesis of MS. Methods Sixty-three patients with MS, 15 cases with optic neuromyelitis (ONM) and 31 cases with other neuropathic inflammatory diseases (OIND) were examined by enzyme-linked immunosorbent assay (ELISA) and 18 other non-inflammatory (NIND), and 46 normal controls (NCs) in the nervous system), NF-L and its antibodies in serum and cerebrospinal fluid, anti-myelin basic protein (MBP), anti-myelin oligodendrocyte glycoprotein ) Antibody level. Normal distribution data with x ~ - s said non-normal distribution of information using M (QR) said. Results The concentration of NF-L in cerebrospinal fluid was 26 (33) ng / L in MS group, 20 (92) ng / L in ONM group and 19 (82) ng / L in OIND group and 30 , And the NC group was 10 (7) ng / L. The concentration of NF-L in cerebrospinal fluid in other groups were significantly higher than those in NC group (all P <0.01). The level of NF-L antibody in MS group was lower than that in OIND group and NIND group and NC group (all P <0.05). The levels of NF-|ÊB in cerebrospinal fluid of MS patients were significantly correlated with the level of MBP antibody (r = 0.784, P <0.01) and IgG level (r = 0.675, P <0.01), but not with MOG antibody level. Conclusion NF-L or its antibody can not be used as an ideal marker to identify various neurological diseases. Axonal injury exists in all groups of MS, ONM, OIND and NIND. The inflammation may not be the result of NF-L antibody, The main reason for injury.