目前,抗体在在临床医学研究和食品环境安全检测等方面应用广泛,随着分子生物学的不断发展,利用基因工程手段获得基因工程抗体的方法成为抗体技术研究的热点,自从Smith于1985年第一次成功地将EcoRⅠ核酸内切酶基因插入丝状噬菌体基因pⅢ中,并在噬菌体表面表达出了融合的蛋白,及1 At present, the antibodies are widely used in clinical medicine research and food environment safety detection. With the continuous development of molecular biology, the methods of obtaining genetically engineered antibodies by genetic engineering have become hot topics in the field of antibody technology. Since Smith in 1985 The EcoRI endonuclease gene was successfully inserted into the filamentous phage pIII and the fusion protein was expressed on the surface of the phage and 1