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目的分析血膜制作过程中影响因素对镜检工作的影响,为提高镜检质量提供帮助。方法采集出国输入疑似疟疾病人的血液标本,制作厚薄血膜、染色、镜检。结果玻片清洁、血量适宜、制作合格的血膜,染液质量,漂洗方法及高水平镜检是发现疟原虫的几个关键因素。结论制作合格的血膜、好的染色质量、正确的漂洗方法及高技术的镜检能力,是提高镜检质量和检出率的有力保障。
Objective To analyze the influence of influencing factors on the microscopic examination in the process of blood film production, and to help improve the quality of microscopic examination. Methods Blood samples were collected from patients who entered the country for suspected malaria. Thick and thin blood films were prepared, stained and examined microscopically. Results Several of the key factors for the detection of Plasmodium were the clean glass slides, adequate blood volume, well-established blood films, dye liquor quality, rinse methods and high-level microscopy. Conclusion The qualified blood film, good dyeing quality, correct rinsing method and high-tech mirror examination ability are the powerful guarantee to improve the quality and detection rate of microscopic examination.