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目的:探讨新清开灵注射液对脑出血模型大鼠血肿周围脑水肿的干预作用。方法:选取100只SPF级雄性SD大鼠,随机选取20只作为假手术组,选取造模成功的60只SD大鼠采用随机数字表法分为模型组及新清开灵低(15 m L/kg)、高(30 m L/kg)剂量组,每组20只,采用免疫组化染色观察血肿周围组织基质金属蛋白酶-9(MMP-9)、组织金属基质蛋白酶抑制剂-1(TIMP-1)表达水平;测定各组大鼠血肿周围脑组织中肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、超氧化物歧化酶(SOD)、丙二醛(MDA)的含量。结果:模型组大鼠脑血肿周围脑组织中MMP-9、TIMP-1表达及TNF-α、IL-1β、MDA含量在术后48、72 h显著高于假手术组,SOD活性显著低于假手术组(P<0.05);新清开灵低、高剂量组脑血肿周围脑组织中MMP-9、TIMP-1表达及TNF-α、IL-1β、MDA含量在术后48、72 h显著低于模型组(P<0.05),SOD活性显著的高于模型组(P<0.05)。模型组大鼠脑含水量在术后72 h显著高于假手术组(P<0.05);新清开灵低、高剂量组脑含水量在术后72 h显著低于模型组(P<0.05)。结论:新清开灵注射液治疗脑出血模型大鼠可减轻脑水肿程度,其作用机制与减轻炎症反应、提高SOD活性、降低MMP-9、TIMP-1表达有关。
Objective: To investigate the intervention effect of Qingkailing Injection on cerebral edema around hematoma in intracerebral hemorrhage model rats. Methods: A total of 100 SPF male Sprague-Dawley rats were randomly divided into sham operation group (n = 20) and 60 SD rats (success model) were randomly divided into model group and new Qingkailing low (15 m L / kg) and high dose (30 m L / kg), 20 rats in each group. The expression of MMP-9 and TIMP-1 were detected by immunohistochemistry -1), and the levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), superoxide dismutase (SOD) (MDA) content. Results: The expression of MMP-9, TIMP-1 and the content of TNF-α, IL-1β and MDA in cerebral tissue around cerebral hematoma in model group were significantly higher than sham operation group at 48 and 72 h after operation (P <0.05). The expression of MMP-9, TIMP-1 and the content of TNF-α, IL-1β and MDA in cerebral tissue around cerebral hematoma of Xinqingkailing low and high dose group were significantly increased at 48 and 72 h Significantly lower than the model group (P <0.05), SOD activity was significantly higher than the model group (P <0.05). The brain water content in the model group at 72 h after operation was significantly higher than that in the sham operation group (P <0.05). The brain water content of the model group was significantly lower at 72 h after operation than that of the model group (P <0.05) ). Conclusion: New Qingkailing injection can reduce cerebral edema in rats with intracerebral hemorrhage. Its mechanism may be related to the reduction of inflammatory reaction, the increase of SOD activity and the decrease of the expression of MMP-9 and TIMP-1.